Email this article Possibilities of investigating intratumoral lymphocyte subpopulations by flow cytometry of tumor core biopsy specimens in patients with breast cancer
- Authors: Shamilov F.A.1, Vishnevskaya Y.V.2, Selchuk V.Y.1, Pogodina E.M.2, Zernov D.I.2, Chkhikvadze N.V.2, Timoshenko V.V.2, Ryabchikov D.A.2, Tupitsyn N.N.2
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Affiliations:
- Department of Oncology, Moscow State University of Medicine and Dentistry
- N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow
- Issue: No 3-4 (2012)
- Pages: 29-33
- Section: MAMMOLOGY. DIAGNOSIS
- Published: 24.09.2012
- URL: https://ojrs.abvpress.ru/ojrs/article/view/111
- DOI: https://doi.org/10.17650/1994-4098-2012-0-3-4-29-33
- ID: 111
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Abstract
Study of tumor-infiltrating lymphocyte subpopulations was performed in 19 patients with breast cancer (core biopsy material). Two probes were used in multiparameter flow cytometry: CD4/CD25/CD3/CD45 and CD8/CD56/CD3/CD45. Mean percentage of different cell types within lymphocytes (CD45++ SSCLow) was as follows: CD3 – 88.2%, CD4 – 45.1%, CD8 – 40.4%, NK (CD3–CD56+) – 1.6%, CD4+CD25+ – 4.1 %. Ratio CD8/CD4 was less than 1 in 53 % of cases. In cases of prominent and moderate CD45 infiltration percentage of CD8+ lymphocytes was significantly higher than in cases with low infiltration, p = 0.007.
About the authors
F. A. Shamilov
Department of Oncology, Moscow State University of Medicine and Dentistry
Author for correspondence.
Email: farhad8282@rambler.ru
Russian Federation
Ya. V. Vishnevskaya
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowRussian Federation
V. Yu. Selchuk
Department of Oncology, Moscow State University of Medicine and DentistryRussian Federation
E. M. Pogodina
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowRussian Federation
D. I. Zernov
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowRussian Federation
N. V. Chkhikvadze
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowRussian Federation
V. V. Timoshenko
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowRussian Federation
D. A. Ryabchikov
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowRussian Federation
N. N. Tupitsyn
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowRussian Federation
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