Possibilities of investigating intratumoral lymphocyte subpopulations by flow cytometry of tumor core biopsy specimens in patients with breast cancer
https://doi.org/10.17650/1994-4098-2012-0-3-4-29-33
Abstract
Study of tumor-infiltrating lymphocyte subpopulations was performed in 19 patients with breast cancer (core biopsy material). Two probes were used in multiparameter flow cytometry: CD4/CD25/CD3/CD45 and CD8/CD56/CD3/CD45. Mean percentage of different cell types within lymphocytes (CD45++ SSCLow) was as follows: CD3 – 88.2%, CD4 – 45.1%, CD8 – 40.4%, NK (CD3–CD56+) – 1.6%, CD4+CD25+ – 4.1 %. Ratio CD8/CD4 was less than 1 in 53 % of cases. In cases of prominent and moderate CD45 infiltration percentage of CD8+ lymphocytes was significantly higher than in cases with low infiltration, p = 0.007.
About the Authors
F. A. Shamilov
Department of Oncology, Moscow State University of Medicine and Dentistry
Russian Federation
Russian Federation
Ya. V. Vishnevskaya
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow
Russian Federation
Russian Federation
V. Yu. Selchuk
Department of Oncology, Moscow State University of Medicine and Dentistry
Russian Federation
Russian Federation
E. M. Pogodina
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow
Russian Federation
Russian Federation
D. I. Zernov
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow
Russian Federation
Russian Federation
N. V. Chkhikvadze
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow
Russian Federation
Russian Federation
V. V. Timoshenko
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow
Russian Federation
Russian Federation
D. A. Ryabchikov
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow
Russian Federation
Russian Federation
N. N. Tupitsyn
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow
Russian Federation
Russian Federation
References
1. Артамонова Е.В. Роль иммунофенотипирования опухолевых клеток в диагностике и прогнозе рака молочной железы. Иммунология гемопоэза 2009;6(1):8−51.
2. Артамонова Е.В., Огнерубов Н.А., Тупицын Н.Н., Летягин В.П. Рак молочной железы: иммунологические факторы прогноза. Издательство Воронежского государственного университета, 2005. 240 с.
3. Субботина А.А., Летягин В.П., Тупицын Н.Н., Ермилова В.Д., Высоцкая И.В. Роль иммунофенотипирования рака молочной железы в процессе неоадъювантной химиотерапии. Иммунология гемопоэза 2009;6(1):52−78.
4. Тупицын Н.Н. Иммунофенотип рака молочной железы. Рак молочной железы. Под ред. Н.Е. Кушлинского, С.М. Портного, К.П. Лактионова. М.: РАМН, 2005. С. 174−97.
5. DeNardo D.G., Drennan D.J., Rexhepaj E. et al. Leucocyte complexity predicts breast cancer survival and functionally regulates response to chemotherapy. Cancer Discovery 2011;1(1):54−67.
6. Ruffel B., Au A., Rugo H.S. et al. Leucocyte composition of human breast cancer. PNAS 2012;109(8):2796−801.
Review
For citations:
Shamilov F.A., Vishnevskaya Ya.V., Selchuk V.Yu., Pogodina E.M., Zernov D.I., Chkhikvadze N.V., Timoshenko V.V., Ryabchikov D.A., Tupitsyn N.N. Possibilities of investigating intratumoral lymphocyte subpopulations by flow cytometry of tumor core biopsy specimens in patients with breast cancer. Tumors of female reproductive system. 2012;(3-4):29-33. (In Russ.) https://doi.org/10.17650/1994-4098-2012-0-3-4-29-33
Views:
579
Email this article 