Analysis of the expression of genes involved in proliferation and apoptosis in cervical intraepithelial neoplasias and cancer of the cervix uteri

The viral nature of many female genital cancers is now beyond question. By taking into account this fact, the problem of qualitative assessment ofthenatureofcervicalintraepithelialneoplasia(CIN)anditsfocusonprogressiontoinvasivecarcinomabecomesquitenatu ral.Studiesof a number of biological markers of carcinogenesis have recently provided a possibility for prospective prediction. The paper con siders the as- pects of importance of the molecular biological markers of proliferation and apoptosis in the etiopathogenesis of genital cancers. It gives the results of examinations of 16 patients with histologically verified squamous cell carcinoma of the cervix uteri, 40 patients di agnosed as having CIN of different grades (CIN-1, CIN-2, CIN-3 — squamous cell carcinoma in situ), and 6 patients with the morphologically unaltered cervi- calepithelium,whosecervicalscrapeswereanalyzedfortheexpressionofthemRNAgenesССNB1,Ki-67,BA G,BCL-2,ESR1,andPRG. It is shown that the molecular genetic findings may be new prognostic markers that reflect the possible disease developmental pathways, sug- gesting the need for further investigation of biomarkers in order to prevent malignancies and to reduce their morbidity.

cancer of the cervix uteri, ССNB1, Ki-67, BAG, BCL-2, ESR1, PRG, human papillomavirus

1. Давыдов М.И., Аксель Е.М. Злокачественные новообразования в России и странах СНГ в 2008 г. М.: РОНЦ им. Н.Н. Блохина, 2010.

2. Злокачественные новообразования в России в 2008 году (заболеваемость и смертность). Под ред. В.И. Чиссова, В.В. Старинского, Г.В. Петровой. М.: МНИОИ им. П.А. Герцена, 2010. http://www.oncology.ru/service/statistics/ morbidity/2008.pdf

3. Zur Hausen H. Papillomaviruses in anogenital cancer as a model to understand the role of viruses in human cancers. Cancer Res 1989;49(17):4677–81.

4. Castle P.E., Fetterman B., Poitras N. at al. Variable risk of cervical precancer and cancer after a human papillomavirus-positive test. Obstet Gynecol 2011;117(3):650–6.

5. Zielinski D.G., Snijders P.J., Rozendaal L. et al. High-risk HPV testing in women with borderline and mild dyskaryosis: long-term follow-up data and clinical relevance. J Pathol 2001;195(3):300–6.

6. Kjær S.K., Frederiksen K., Munk C., Iftner T. Long-term absolute risk of cervical intraepithelial neoplasia grade 3 or worse following human papillomavirus infection: role of persistence. J Natl Cancer Inst 2010;102(19):1478–88.

7. Koskimaa H.M., Kurvinen K., Costa S. et al. Molecular markers implicating early malignant events in cervical carcinogenesis. Cancer Epid Biomarker Prev 2010;19(8):2003–12.

8. Tang S.C. BAG-1, an anti-apoptotic tumour marker. IUBMB Life 2002;53(2):99–105.

9. Saha B., Chaiwun B., Tsao-Wei D.D. et al. Telomerase and markers of cellular proliferation are associated with the progression of cervical intraepithelial neoplasia lesions. Int J Gynecol Pathol 2007;26(3):214–22.

10. Rajkumar T., Sabitha K., Vijayalakshmi N. et al. Identification and validation of genes involved in cervical tumourigenesis. BMC Cancer 2011;11:80.