IMMUNOHISTROCHEMISTRY AND FLOW CYTOMETRIC CHARACTERISTICS OF INTRATUMORAL LYMPHOCYTES IN OVARIAN SEROUS ADENOCARCINOMA
https://doi.org/10.17650/1994-4098-2009-0-3-4-117-121
Abstract
Intratumoral T-lymphocyte subpopulations were studied in 27 patients with ovarian serous adenocarcinoma, by using immunohisto- chemical techniques (n = 27) and three-color flow cytometry (n = 11).
The subpopulations of intratumoral lymphocytes infiltrating the tumor parenchyma and stroma differed. The intraepithelial cells were mainly CD8+ T cells (30.7%) and CD56+ lymphocytes (25%), which were indicative of the predominance of NK cells. Stromal infil- tration was primarily presented by mature CD3+ T lymphocytes (33%). This was supported by flow cytometric studies; CD3+ constitut- ed 62—92.6% among the CD45+ intratumoral leukocytes. The CD3+CD8+/CD3+CD4+ ratio was > 2 only in 3 cases. CD25+ cells made up the bulk among the CD3+CD4+ lymphocytes - 30—60% in 6 patients
About the Authors
E. V. Zuyeva
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow
Russian Federation
Russian Federation
S. O. Nikogosyan
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow
Russian Federation
Russian Federation
V. V. Kuznetsov
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow
Russian Federation
Russian Federation
A. V. Mozhenkova
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow
Russian Federation
Russian Federation
N. V. Kokosadze
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow
Russian Federation
Russian Federation
V. V. Timoshenko
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow
Russian Federation
Russian Federation
M. Zh. Karibayeva
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow
Russian Federation
Russian Federation
N. N. Tupitsyn
N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow
Russian Federation
Russian Federation
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Review
For citations:
Zuyeva E.V., Nikogosyan S.O., Kuznetsov V.V., Mozhenkova A.V., Kokosadze N.V., Timoshenko V.V., Karibayeva M.Zh., Tupitsyn N.N. IMMUNOHISTROCHEMISTRY AND FLOW CYTOMETRIC CHARACTERISTICS OF INTRATUMORAL LYMPHOCYTES IN OVARIAN SEROUS ADENOCARCINOMA. Tumors of female reproductive system. 2009;(3-4):117-121. (In Russ.) https://doi.org/10.17650/1994-4098-2009-0-3-4-117-121
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