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EVALUATION OF EXPRESSION OF 4 MIRNAS IN CYTOLOGICAL SAMPLES AS AN ADDITIONAL METHOD OF CERVICAL CANCER DIAGNOSIS
- Authors: Arkhangelskaya P.A.1,2, Samsonov R.B.1,3, Shtam T.A.1,3, Knyazeva M.S.1,3, Ivanov M.K.4,5, Titov S.E.4,5, Kolesnikov N.N.4, Bakhidze E.V.1,2, Berlev I.V.1,2, Mikhetko A.А.1, Vorobyev S.L.6, Malek A.V.1,3
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Affiliations:
- N.N. Petrov National Medical Research Oncology Center, Ministry of Health of Russia
- North-Western State Medical University named after I. I. Mechnikov, Ministry of Health of Russia
- Oncosystem
- Institute of Molecular and Cellular Biology of the Siberian Branch of the Russian Academy of Sciences
- Vector-Best Ltd
- National Centre of Clinical and Morphological Diagnostics
- Issue: Vol 13, No 3 (2017)
- Pages: 63-72
- Section: GYNECOLOGY. ORIGINAL REPORTS
- Published: 03.09.2017
- URL: https://ojrs.abvpress.ru/ojrs/article/view/552
- DOI: https://doi.org/10.17650/1994-4098-2017-13-3-63-72
- ID: 552
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Abstract
Introduction. Cervical cancer is the 4th most common cancer among women. The main screening method for cervical cancer is cytological examination of the cervical epithelium. This method allows to evaluate the level of cervical dysplasia (malignant potential) but it has several limitations and flaws. Development and implementation of new methods of molecular and genetic analysis in clinical practice can increase informational value of the traditional cytological examination and therefore objectivity in choosing treatment options.
Objective is to develop and verify a new method of differential diagnosis of severe intraepithelial dysplasia and invasive cervical cancer.
Materials and methods. The method is based on analysis of small non-coding RNA molecules (miRNAs) extracted from the material of traditional Pap smears. Based on literature search, 18 “marker” microRNA molecules were chosen and their expression levels were estimated in 166 samples of Pap smears from cervical canals with different cytological diagnoses. The analysis was performed using reverse transcription polymerase chain reaction.
Results. Estimation of ratios between expression levels of miRNA pairs: 126/375; 20а/375; 126/145 allows to differentiate with high confidence borderline states of severe intraepithelial dysplasia and invasive cervical carcinoma (coefficients of quantitative interpretation of the error curve were 0.8, 0.75, 0.72, respectively).
Conclusions. Analysis of miRNAs in Pap smear samples is a promising additional method of cervical cancer diagnosis. The method is objective and can be proposed as a supporting technique in cases when cytological examination doesn’t allow to differentiate between borderline pathological states of the cervical epithelium. Implementation of the method in clinical practice requires methodological optimization and additional validation using more clinical material.
About the authors
P. A. Arkhangelskaya
N.N. Petrov National Medical Research Oncology Center, Ministry of Health of Russia; North-Western State Medical University named after I. I. Mechnikov, Ministry of Health of Russia
68 Leningradskaya St., Pesochnyi Settlement, Saint Petersburg 197758; 41 Kirochnaya St., Saint Petersburg 191015
Russian FederationR. B. Samsonov
N.N. Petrov National Medical Research Oncology Center, Ministry of Health of Russia; Oncosystem
Author for correspondence.
Email: Rom_207@mail.ru
68 Leningradskaya St., Pesochnyi Settlement, Saint Petersburg 197758; Room 16, 4–9 Lugovaya St., Skolkovo Innovation Center, Moscow, 143026
Russian FederationT. A. Shtam
N.N. Petrov National Medical Research Oncology Center, Ministry of Health of Russia; Oncosystem
68 Leningradskaya St., Pesochnyi Settlement, Saint Petersburg 197758; Room 16, 4–9 Lugovaya St., Skolkovo Innovation Center, Moscow, 143026
Russian FederationM. S. Knyazeva
N.N. Petrov National Medical Research Oncology Center, Ministry of Health of Russia; Oncosystem
68 Leningradskaya St., Pesochnyi Settlement, Saint Petersburg 197758; Room 16, 4–9 Lugovaya St., Skolkovo Innovation Center, Moscow, 143026
Russian FederationM. K. Ivanov
Institute of Molecular and Cellular Biology of the Siberian Branch of the Russian Academy of Sciences; Vector-Best Ltd
Acad. Lavrentiev Ave. 8/2 Novosibirsk, 630090; ABK zone, Koltsovo Settlement, Novosibirsk District 630559
Russian FederationS. E. Titov
Institute of Molecular and Cellular Biology of the Siberian Branch of the Russian Academy of Sciences; Vector-Best Ltd
Acad. Lavrentiev Ave. 8/2 Novosibirsk, 630090; ABK zone, Koltsovo Settlement, Novosibirsk District 630559
Russian FederationN. N. Kolesnikov
Institute of Molecular and Cellular Biology of the Siberian Branch of the Russian Academy of Sciences
Acad. Lavrentiev Ave. 8/2 Novosibirsk, 630090
Russian FederationE. V. Bakhidze
N.N. Petrov National Medical Research Oncology Center, Ministry of Health of Russia; North-Western State Medical University named after I. I. Mechnikov, Ministry of Health of Russia
68 Leningradskaya St., Pesochnyi Settlement, Saint Petersburg 197758; 41 Kirochnaya St., Saint Petersburg 191015
Russian FederationI. V. Berlev
N.N. Petrov National Medical Research Oncology Center, Ministry of Health of Russia; North-Western State Medical University named after I. I. Mechnikov, Ministry of Health of Russia
68 Leningradskaya St., Pesochnyi Settlement, Saint Petersburg 197758; 41 Kirochnaya St., Saint Petersburg 191015
Russian FederationA. А. Mikhetko
N.N. Petrov National Medical Research Oncology Center, Ministry of Health of Russia
68 Leningradskaya St., Pesochnyi Settlement, Saint Petersburg, 197758
Russian FederationS. L. Vorobyev
National Centre of Clinical and Morphological Diagnostics
8/2 (A) Oleko Dundich St., Saint Petersburg 192283
Russian FederationA. V. Malek
N.N. Petrov National Medical Research Oncology Center, Ministry of Health of Russia; Oncosystem
68 Leningradskaya St., Pesochnyi Settlement, Saint Petersburg 197758; Room 16, 4–9 Lugovaya St., Skolkovo Innovation Center, Moscow, 143026
Russian FederationReferences
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